Scope of Application
【Operating Instructions】
**YG-C610H Fluorescence Quantitative Gene Amplifier (Real-Time PCR System)**
**【Technical Parameters】**
1. **Sample capacity:** 96-well module; compatible with 0.2 mL single tubes, 8‑tube strips, etc.
2. **Recommended optimal reaction volume:** 10–100 µL
3. **Temperature accuracy of the reaction module:** ±0.25 °C
4. **Temperature control method:** Heating via Peltier effect system or semiconductor; cooling
via semiconductor technology
5. **Standard configuration:** High‑speed reaction module; maximum ramp rate: 5 °C/s
6. **Temperature uniformity of the reaction module:** ±0.4 °C
7. **Temperature gradient function:** Simultaneously optimizes up to 6 temperature points for
rapid reaction condition optimization
8. **Optical system:**
- Standard: 4‑detection‑channel module for simultaneous 4‑color fluorescence detection; the instrument
has reserved slots for upgrading to a maximum of 6 detection channels, or can be equipped with
a 6‑detection‑channel module for simultaneous 6‑color fluorescence detection
9. **Detector:** High‑sensitivity photomultiplier tube (PMT), photodiode (PN/PIN), or low‑temperature
high‑resolution CMOS
10. **Light source:** 6 excitation channels
11. **Excitation spectrum range:** 450–730 nm
12. **Detection linear range:** 10 orders of magnitude
13. **Detection sensitivity:** Capable of detecting single‑copy DNA templates
14. **Multiplex data analysis:** Standard software can simultaneously analyze fluorescence data from
at least 4 detection channels
15. **Data analysis modes:** Standard curve quantification, melting curve analysis, ΔCT or ΔΔCT gene
expression analysis, allele analysis, amplification‑efficiency‑based data analysis, and other data analysis functions
16. **Supported fluorescent dyes:** FAM/SYBR, VIC/TET/HEX, ROX/Texas Red, Cy5, and dyes with similar spectra
17. **Display:** Built‑in 8.5‑inch large color touch screen with touchpad; real‑time fluorescent quantitative
PCR experiments can be viewed
18. **High‑resolution melt (HRM) curve analysis function supported:** Melting curve resolution 0.1 °C
19. **Data export:** Excel, Word, or PowerPoint. User reports include run settings, graphical and tabular
results, and can be printed or saved directly
20. **Control and analysis software available in Chinese, English, and other languages, freely switchable;
interface is concise and intuitive
21. **Consumables and reagent kits for the instrument are open platform;** users can choose multiple
consumables and reagents from the original manufacturer as well as from other suppliers on the market.